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申晓慧,姜成,李如来,等.不同诱变处理对紫花苜蓿生理生化指标的影响[J].农学学报,2018,8(10):70-75.姜成,李如来,et al.Effect of Mutagens on Physiological and Biochemical Indexes of Alfalfa[J].Journal of Agriculture,2018,8(10):70-75
不同诱变处理对紫花苜蓿生理生化指标的影响
Effect of Mutagens on Physiological and Biochemical Indexes of Alfalfa
投稿时间:2017-08-09  修订日期:2017-10-17
DOI:10.11923/j.issn.2095-4050.cjas17080011
中文关键词: 诱变  紫花苜蓿  生理生化指标
英文关键词: mutation, alfalfa, physiological  and biochemical  indexes
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基金项目:黑龙江省政府博士后资助项目(LBH-Z12239);中国博士后科学基金(2013M541423)。
作者单位E-mail
申晓慧 黑龙江省农业科学院佳木斯分院 xiaohuishen@126.com 
姜成 佳木斯大学 jiang780920@126.com 
李如来 黑龙江省农业科学院佳木斯分院 rulai@126.com 
冯鹏 黑龙江省农业科学院佳木斯分院 fenggras@163.com 
李增杰 黑龙江省农业科学院佳木斯分院 zengjie@163.com 
李志民 黑龙江省农业科学院佳木斯分院 zhi@126.com 
王强 黑龙江省农业科学院作物育种研究所 wang@163.com 
中文摘要:
      为了比较研究不同诱变处理方式对紫花苜蓿诱变效果的影响。试验通过60Co-γ射线、紫外线、甲基磺酸乙酯(EMS) 3 种诱变处理对供试4 种紫花苜蓿干种子进行诱变,以当代材料为研究对象,探讨诱变处理对苜蓿生理生化指标的影响。诱变处理后,3 种抗氧化酶活性以增加趋势为主。其中对SOD的影响,60Co-γ表现为促进作用,紫外线处理降低了SOD活性,EMS处理表现为低促髙抑作用;3 种诱变处理对POD和CAT的影响为60Co-γ辐射随处理剂量增加酶活性下降,紫外线处理则随着剂量增加酶活性增加,EMS处理则因品种不同而不同。诱变处理对苜蓿可溶性蛋白、可溶性糖和游离脯氨酸含量的影响随剂量及品种变化而不同,对MDA含量影响不显著。不同诱变处理显著提高了苜蓿叶片抗氧化酶活性,其中150 Gy 60Co-γ、90 min紫外线和0.4% EMS处理对苜蓿生理活性具有明显提高作用。
英文摘要:
      This study aims at comparing the effects of different mutagenesis treatments on alfalfa. 60Co-γ rays, ultraviolet and EMS were used for induced mutation of four alfalfa cultivars’dry seeds. The contemporary materials were taken as research objects, the influence of mutagenic treatment on the physiological and biochemical indexes of alfalfa was explored. After mutation treatments, the activity of three anti-oxidative enzymes showed an increasing trend. The effect of 60Co-γ irradiation on SOD was accelerating, ultraviolet treatment reduced SOD activity, EMS treatment on SOD showed low- concentration promotion and highconcentration inhibitory. Along with the increasing of 60Co-γ irradiation, POD and CAT activity decreased, while POD and CAT activity increased with the increasing ultraviolet dosage. The effect of EMS treatment was different in different varieties. The effects of mutagenic treatments on soluble protein, soluble sugar and free proline in alfalfa were different due to dosage and variety, and did not affect MDA content obviously. The mutation treatments improved the activities of three anti-oxidative enzymes, 150 Gy 60Co-γ, 90 min ultraviolet and 0.4% EMS treatment had significant effect on the physiological activity of alfalfa.
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