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徐志伟,徐一然,陈永霞,等.优化ApoB抗原免疫剂量提高抗体产生的效果[J].农学学报,2018,8(11):58-61.,et al.Optimization of ApoB Antigen Immunizing Dose for Improving Antibody Production Efficiency[J].Journal of Agriculture,2018,8(11):58-61
优化ApoB抗原免疫剂量提高抗体产生的效果
Optimization of ApoB Antigen Immunizing Dose for Improving Antibody Production Efficiency
投稿时间:2017-08-22  修订日期:2017-11-28
DOI:10.11923/j.issn.2095-4050.cjas17080020
中文关键词: 湖羊  ApoB抗原  抗体效价。
英文关键词: Hu sheep  ApoB antigen  antibody titer  ApoB production performance
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基金项目:江苏省农业科技自主创新资金项目[CX(16)1054]。
作者单位E-mail
徐志伟 江苏丘陵地区镇江农业科学研究所江苏句容 xzw1729@sina.com 
徐一然 江苏大学江苏镇江 1281773306@qq.com 
陈永霞 江苏省句容市动物疫病预防控制中心江苏句容 jsjrcyx1139@163.com 
刘伟忠 江苏丘陵地区镇江农业科学研究所江苏句容 liuweizhong168@126.com 
徐小波 江苏省农业科学院畜牧所江苏南京 xiaoboxu@sohu.com 
于建宁 江苏省农业科学院畜牧所江苏南京 jianningyu@gmail.com 
中文摘要:
      以湖羊为研究对象,设计了3种不同的ApoB抗原免疫剂量,从抗体产生规律、抗体效价和ApoB产品性能三个方面进行了统计和分析。结果表明:组2(抗原剂量每次0.4 mg)的抗体效价显著高于组1(抗原剂量每次0.2 mg)和组3(抗原剂量每次1 mg),组2五免后的抗体效价能够达到1:29,而且ApoB的产品性能检测各个指标均合格。表明ApoB抗原每次免疫注射剂量为0.4 mg时,所生产的ApoB抗体效果最佳,为ApoB抗体广泛应用于医疗领域奠定基础。
英文摘要:
      With Hu sheep as the research object, we designed three different doses of ApoB antigens. The antibody production, antibody titer and ApoB product performance were analyzed. The results showed that the antibody titer of group 2 (0.4 mg antigen each time) was significantly higher than those of group 1 (0.2 mg antigen each time) and that of group 3 (1 mg antigen each time). Antibody titer could reach 1:29 after five times of immunization in group 2. The indexes of ApoB production performance were qualified, which indicated that the best efficiency of ApoB antibody was produced when each immunization antigen was 0.4 mg ApoB antigen. This study laid a good foundation for the wide utilization of ApoB antibody in medical field.
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